Conferences

American Society for Veterinary Clinical Pathology
2424 American Lane
Madison, WI 53704

Telephone: +1-608-443-2479
Fax: +1-608-443-2474
email: info@asvcp.org

2005 Young Investigator Award Winner, Boston, Mass.

Roberta Di Terlizzi (Kansas State University) is congratulated by Past-President Harold Tvedten upon receiving the 2005 ASVCP Young Investigator AwardRoberta Di Terlizzi (Kansas State University) is congratulated by Past-President Harold Tvedten upon receiving the 2005 ASVCP Young Investigator Award

Roberta Di Terlizzi (Kansas State University) is congratulated by Past-President Harold Tvedten upon receiving the 2005 ASVCP Young Investigator Award for her research presentation, "Comparison of protein concentrations in precolostral and postcolostral bovine sera using spectrophotmetric, refractometric, electrophoretic, and radioimmunodiffusion methods". Dr. Di Terlizzi received a check for $500. Her abstract is presented below:

COMPARISON OF PROTEIN CONCENTRATIONS IN PRECOLOSTRAL AND POSTCOLOSTRAL BOVINE SERA USING SPECTROPHOTOMETRIC, REFRACTOMETRIC, ELECTROPHORETIC, AND RADIOIMMUNODIFFUSION METHODS.

R. Di Terlizzi, S.L. Stockham, R. Kempegowda, M.J. Wilkerson. Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA.

The objective of this study was to compare protein concentrations in precolostral and postcolostral bovine sera using spectrophotometric, refractometric, electrophoretic, and radioimmunodiffusion methods. Serum samples were collected from 30 newborn Holstein calves prior to and 24 hours after ingestion of colostrum (1-2 feedings, 2 qt. for each feeding). Also sera were obtained from 8 healthy 2-3 months old male castrated calves and were pooled to create control serum. Calf samples and control serum aliquots were analyzed in duplicate. Total protein concentrations were determined using two different methods: biuret reaction (Hitachi 911) and refractive index using two refractometers (Leica VET 360 and Leica TS 400). Total globulin concentrations were calculated by subtracting albumin concentrations (bromcresol green) from biuret total protein concentrations. Serum protein fractions were separated by electrophoresis using Titan III cellulose acetate membranes (CA) and Titan Gel system (TG), stained with Ponceau S and amido black stains (respectively), and scanned using Quick Scan 2000 Windows®. Total immunoglobulin G (IgG) concentrations were determined by radioimmunodiffusion (RID, Immunocheck Bovine IgG RID kit, 0.4-3.2 g/dL). By all comparison methods, measured IgG concentrations had an unacceptable positive proportional bias (Deming regression). The differences (postcolostral minus precolostral) in total protein concentrations and differences in the total globulin concentrations compared to differences in γ-globulin concentrations (both CA and TG) had a positive proportional biases (Deming regression). Differences in γ-globulin concentrations determined by CA and TG methods had good agreement (average bias < 0.1 g/dL). These results indicate that γ-globulin concentration determined by electrophoresis was the best of the evaluated methods for assessing increased IgG concentration after ingestion of colostrum.